Ubiquitination independent of E1 and E2 enzymes by bacterial effectors
نویسندگان
چکیده
منابع مشابه
Protein ubiquitination and formation of polyubiquitin chains without ATP, E1 and E2 enzymes.
Studying protein ubiquitination is difficult due to the complexity of the E1-E2-E3 ubiquitination cascade. Here we report the discovery that C-terminal ubiquitin thioesters can undergo direct transthiolation with the catalytic cysteine of the model HECT E3 ubiquitin ligase Rsp5 to form a catalytically active Rsp5∼ubiquitin thioester (Rsp5∼Ub). The resulting Rsp5∼Ub undergoes efficient autoubiqu...
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The proposal that some naturally occurring prostaglandins (PGs) or their by-products may be implicated in the pathogenesis of the asthmatic bronchospasm has been suggested. Other PGs may be potentially useful in the treatment of this lung disease. The present investigation compared the bronchodilator effects of PGE1 and PGE2 in pharmacologically constricted experimental animals. In pentobarbita...
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Acknowledgements Acknowledgements 2 First of all I would like to thank Prof. Marcus Groettrup for giving me the opportunity to perform my PhD in his group. Thank you for supplying optimal working conditions and providing scientific guidance over the past years. Thank you to Prof. Martin Scheffner's group for the collaboration in the UBE1L2 paper and for lots of advice. I would like to express m...
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Protein modification by ubiquitin (UB) controls diverse cellular processes. UB is conjugated to cellular proteins by sequential transfer through an E1-E2-E3 enzymatic cascade. The cross-activities of 2 E1s, 50 E2s and thousands of E3s encoded by the human genome make it difficult to identify the substrate proteins of a specific E3 enzyme in the cell. One way to solve this problem is to engineer...
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2014 We investigate the possibility to observe Doppler-free two-photon transitions in the case where one of the transitions is an electric dipole transition and the other one is a magnetic dipole or an electric quadrupole transition. We show that it is not possible to obtain Doppler-free lines by reflecting the beam on itself. On the other hand, such an effect should be observable if the two co...
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ژورنال
عنوان ژورنال: Nature
سال: 2016
ISSN: 0028-0836,1476-4687
DOI: 10.1038/nature17657